Technical Information
Access comprehensive technical resources, procedures, and instructions for optimal use of RDO products.
Product Overview
Both RDO and RDO Gold are unsurpassed decalcifiers that can be tailored to suit your specific lab routine. As with most acids, nucleic acids in the cell can become subject to ribonuclease digestion, resulting in a loss of basophilic properties.
Careful monitoring should be used with RDO to avoid over decalcification. Decalcification will depend on the thickness and density of specimens. Overnight decalcification should be avoided.
Full-strength RDO is used for hard compact bone (e.g., femur heads). Both RDO and RDO Gold may be diluted with tap water to permit multipurpose use. Dilution with tap water is recommended for mildly calcified specimens such as bone marrow biopsies (core).
In either case, the standard procedure should be to check the specimen periodically to determine the endpoint of decalcification.
Key Technical Considerations
RDO Decalcifier, RDO Gold Decalcifier and RDO Lab Grade Decalcifier can be tailored to suit your specific lab routine.
Check specimens periodically to avoid over decalcification and preserve morphology.
Decalcification depends on specimen thickness and density—avoid overnight exposure.
Use full strength for hard compact bone; dilute for mildly calcified specimens when appropriate.